The different ties that bind

The different ties that bind B efore they separate into daughter cells during mitosis, sister chroma-tids are held together by cohesin complexes. But these complexes aren't the same along the entire length of the chro-matids, according to Canudas and Smith: The version of cohesin that links sister telomeres is different from the form that fastens centromeres together (1). Cohesin consists of four subunits, three of which (Smc1, Smc3, and Scc1) form a ring associated with the fourth subunit, Scc3. In vertebrate cells, Scc3 comes in two, slightly different versions , called SA1 and SA2. It's not clear why vertebrate cells express both of these variants—one possibility is that they have different functions. In 2007, Silvia Canudas, Susan Smith, and their colleagues at the New York University School of Medicine found that, unlike SA2, SA1 interacts with a telomere protein called TIN2. Cells unable to separate their telomeres during mitosis are rescued by knocking down SA1, suggesting that SA1 might be specifi cally involved in telomere cohesion (2). Canudas and Smith therefore took a closer look at the chromosomes of cells lacking SA1, using fl uorescent probes to different regions of sister chromatids to determine how closely they were held together. Cohesion between sister telomeres was prematurely lost in the absence of SA1, and chromatid arms drifted further apart, too. Sister chromatids remained attached at their centromeres, however. The situation was reversed in cells missing SA2—sister centromeres were prematurely separated while chromatid arms and telomeres retained their association. Telomere and centromere cohesion are thus regulated by different versions of the cohesin complex, containing either SA1 or SA2, respectively. Why should these chromosomal regions be held together in different ways? Smith speculates that some of the unique features of telomere replication might require cohesion to be regulated differently at chromatid ends. Knocking down SA1's telomeric binding partner TIN2 also caused chro-matid arms to separate, indicating that telomere cohesion is particularly important for sister chromatid attachment. Smith is excited by this unexpected fi nd-ing: " There must be something special happening at telomeres for it to have such a dramatic infl uence on cohesion. " Telomeric cohesin complexes containing SA1 and TIN2 might help stabilize cohesins on chromatid arms, preventing them from slipping off the chromosome ends. Sister telomere cohesion is important for other reasons, too. Canudas and Smith found that cells lacking either SA1 or TIN2 were unable to repair double-stranded DNA …